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B.Sc II Syllabus

B.Sc II Syllabus

                          B.Sc.II 
Fundamentals of Biochemistry

B.Sc.-II Sem-III : Major Core : DSC1-T6 : Fundamentals of Biochemistry (24MJ37133) Cr 2

Hours 30


Learning Outcomes: To study about the classification, properties and significance of Carbohydrates, Lipids. properties of amino acids. To study about the different levels of structural organizations of Proteins. To understand the classification and working mechanism of Enzymes. To study the types of antibodies and their significance.



Unit 1

Carbohydrates                                                                                       

  • Classification of Carbohydrates

  • Structure and examples of Monosaccharides

  • Properties of monosaccharides.

  • Structure and examples of Disaccharides

  • Types, nature and formation of Glycosidic bond

  • Reducing and non-reducing sugars

  • Structure and examples of Polysaccharides- Homo polysaccharides and Hetero polysaccharides

  • Biological Significance carbohydrates 

6

Unit 2

Lipids   

  • Classification of lipids

  • General properties of lipids 

  • Saturated and unsaturated fatty acids

  • Triacylglycerol, Phospholipids, Cholesterol , Steroids

  • Lipid Profile: HDL, LDL, Ratios

  • Biological Significance of lipids.                                                                                                                             

6

Unit 3

Amino Acids and Proteins 

Amino Acids 

  • Amino acids: Structure

  • Classification of Amino acids based on R group and it’s properties

  • General Properties of amino acids

  • Essential and non essential amino acids

Proteins

  • Primary structure of proteins: formation of polypeptide chain in primary structure 

  • Secondary structure with alpha helix, beta sheets (Parallel and anti-parallel) 

  • Tertiary & quaternary structure of proteins 

  • Stabilizing forces of proteins: Covalent interactions – Peptides bonds and Disulfide links 

  • Non-covalent interactions- Hydrogen Bond, Hydrophobic interactions, Van Der Waals Interactions, Electrostatic or ionic bond or salt bond or salt bridge, Dipole movements 

  • Simple and conjugate proteins with their significance

10

Unit 4

Enzymes  

  • Nomenclature and classification

  • Co-factors                                                                                                      

  • Properties of enzymes

  • Mechanism of enzyme action 

  • Factors affecting enzyme actions

  • Isoenzyme

Immunoglobulins

  • Basic Structure. 

  • Types of immunoglobulins

  • Biological significance of antibodies

8

B.Sc.-II Sem-III : Major Core : DSC1- P4: Practical based on DSC1-T6 (24MJ37134) Cr 2

Experiment

Title

1

Qualitative analysis of carbohydrates (Glucose, Fructose, Lactose, Maltose, Sucrose and starch).

2

Identification tests for Proteins (albumin and Casein).

3

Biochemical tests of samples containing lipids.

4

Quantitative analysis of reducing sugars (DNSA method)

5

Quantitative analysis of Proteins (Biuret method).

6

Paper chromatography of isolation of amino acids from mixed sample.

7

Identification of unknown amino acids through paper chromatography.

8

Study of Anti serum ABD and ABO blood group identification.

9

Action of Salivary amylase under optimum conditions.

10

Effect of PH on action of salivary amylase

11

Effect of temperature on the action of salivary amylase.

12

Preparation of buffer solution and measurement of pH.- 04,07 and 09.

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